Objective To monitor and identify the ticks carried by wool imported from Kyrgyz Republic to China, and to prevent the transmission of harmful vectors through international trade. Methods Sixteen batches of wool imported from Kyrgyz Republic at Turgart port, Xinjiang Uygur Autonomous Region, from September 2017 to June 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene, 18S rRNA gene, and cytochrome C oxidase subunitⅠgene were amplified and sequenced to construct a phylogenetic tree using MEGA 7.0 software for molecular biological identification. Results Sixteen batches of wool were imported from Kyrgyz Republic, and ticks were detected in 11 batches of the imported wool. A total of 39 ticks were detected, including 1 live tick. They belonged to 5 species of 4 genera in 2 families as identified by morphological and molecular phylogenetic analysis: Ornithodoros lahorensis, Hyalomma asiaticum, Dermacentor marginatus, Haemaphysalis punctata, and H. sulcata. There were 30 ticks of H. sulcata, as the predominant species (76.9% of the total number) of intercepted ticks carried by the wool imported from Kyrgyz Republic. The live tick was a nymph of O. lahorensis. Conclusion The intercepted ticks are probably detected in wool imported from Kyrgyz Republic, which are mainly H. sulcata. The live nymph of O. lahorensis was intercepted for the first time. Therefore, the quarantine of ticks carried by wool imported from Kyrgyz Republic should be strengthened to prevent vector invasion and transmission of harmful diseases.

Objective To quarantine and identify the ticks carried by wool imported from South Africa to China, and to prevent the transmission of harmful parasites through international trade. Methods Thirty-one batches of wool imported from South Africa at Shanghai port from January 2016 to August 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene and cytochrome C oxidase subunit I gene were amplified and sequenced for molecular biological identification. Results Thirty-one batches of wool were imported from South Africa at Shanghai port from January 2016 to August 2018. Ticks were observed in 8 batches of the imported wool, with a positive detection rate of 25.8%. They belonged to 8 species and 3 genera as identified by morphological and molecular biological methods. There were 15 ticks of Rhipicephalus evertsi evertsi, as the predominant species (53.6% of the total number) of intercepted ticks carried by the wool imported from South Africa; there was no distribution record of this tick species in China. Conclusion The intercepted ticks carried by wool imported from South Africa are mainly Rh. e. evertsi, which can transmit multiple animal diseases including babesiosis.

Objective To identify the unknown species of ticks carried by the dog imported from Germany to China. Methods Use morphological identification, and extract the genomic DNA from the ticks, amplify the mitochondrial 16S rRNA sequences and sequencing. Blast in the NCBI, to identify the species of the ticks collected from Shanghai port on November 28, 2016. Results Morphological analysis revealed that the tick was in accordance with the morphological characteristics of Haemaphysalis. The sequence analysis results showed that the measured gene of this tick was 98% identical to the mitochondrial gene sequence of Ha. flava Neumann, 1897 which was widely distributed in Palaearctic and Oriental region. Conclusion This tick is Ha. flava, and infected the dog after entry.

This study reports one new record of the genus Fannia in Shanghai, China: Fannia dupla Nishida, 1974. This new fly species was cage-trapped in the green belt of Huaihai park of Shanghai and was considered migrating from the outside of Shanghai area. The F. dupla was recorded only in Japan and Taiwan province of China before, but in recent years, it was reported also in Beijing and Fujian province, besides, we found this fly species in 2 consecutive years in the same area of Shanghai (2012 and 2013). The present paper deals with the diagnostics, and the possible biology, distribution of this particular species．

Objective To monitor the species composition and seasonality of calyptrate flies in four types of habitats in Yangpu district, Shanghai, China, and to provide a scientific strategy for fly control. Methods Cage traps were set up in the following four types of habitats: large-scale greenbelts (parks), greenbelts around restaurants, residential areas, and farm produce markets. Results A total of 1588 flies, belonging to 5 families, 25 genera, and 39 species, were captured from 2010 to 2013. The predominant strains were Muscina stabulans, Lucilia sericata, Aldrichina grahami, Anothomyia illocata, Chrysomya megacephala, and Muscina angustifrons. The seasonality showed peaks in June and September and the lowest population density in 2010. In the four types of habitats, the large-scale greenbelts showed the highest population diversity: 32 species, and the highest density was found in the farm produce markets with an average of 4.70 flies/cage·day. Conclusion The populations of calyptrate flies are abundant in habitats of Yangpu district, Shanghai. The predominant populations such as M. stabulans, L. sericata, and A. grahami should be the focus of fly control. Primary control measures should be taken in farm produce markets, and in June and September as the key time points for the prevention and control of fly-transmitted diseases.

Objective To monitor the species composition and seasonality of calyptrate flies in four types of habitats in Yangpu district, Shanghai, China, and to provide a scientific strategy for fly control. Methods Cage traps were set up in the following four types of habitats: large-scale greenbelts (parks), greenbelts around restaurants, residential areas, and farm produce markets. Results A total of 1588 flies, belonging to 5 families, 25 genera, and 39 species, were captured from 2010 to 2013. The predominant strains were Muscina stabulans, Lucilia sericata, Aldrichina grahami, Anothomyia illocata, Chrysomya megacephala, and Muscina angustifrons. The seasonality showed peaks in June and September and the lowest population density in 2010. In the four types of habitats, the large-scale greenbelts showed the highest population diversity: 32 species, and the highest density was found in the farm produce markets with an average of 4.70 flies/cage·day. Conclusion The populations of calyptrate flies are abundant in habitats of Yangpu district, Shanghai. The predominant populations such as M. stabulans, L. sericata, and A. grahami should be the focus of fly control. Primary control measures should be taken in farm produce markets, and in June and September as the key time points for the prevention and control of fly-transmitted diseases.

Objective To get the morphological characterization and other identification data of Calliphora coloradensis intercepted at the Shanghai port. Methods Taxonomic studies was performed. Results Brief description of main morphological characteristics and illustration for the male aedeagus were made based on the intercepted specimen of C. coloradensis. The characteristics of 13 Calliphoraspecies distributed in North America were retrieved. Conclusion Flies can be imported by empty containers. The morphological characteristics and the key can be used as references in the identification and classification of interceptedCalliphoraspecies.

Objective To investigate the species and distributions of fly-borne pathogens in Shanghai Pudong International Airport. Methods The bacteria on the body surfaces and inside the bodies of flies were isolated and cultured. The monoclonal strains were subject to automatic analysis using Vitek 2 Compact and 16S rRNA sequence alignment. Results Eighteen species of bacteria (2 pathogens and 14 opportunistic pathogens including Wohlfahrtiimonas chitiniclastica that had not been reported in China) were detected on the body surfaces of 7 species of flies, Fourteen species of bacteria (1 pathogens and 12 opportunistic pathogens) were detected inside the bodies of the flies. The fly that carried the most species of bacteria was Sarcophaga misera, followed by Lucilia illustris. Conclusion The investigation of fly-borne pathogens in port will be in favor of the identification of new pathogen species and response to public health emergencies.

Objective To investigate the population composition and seasonal dynamics of Calyptrate flies in Yangpu district, Shanghai. Methods The cage trap and butterfly nets Methods were used to capture the flies. Results Sixty-six species (4 families, 41 genus) were collected in the area from March, 2006 to December, 2009. The dominant species was Chrysomya megacephala, accounting for 76.67%. The flies first appeared in March and gradually disappeared in December. Two peaks of the population were observed in August and November and the highest density was in November. Conclusion The fly population was abundant in this district with Ch. megacephala as the dominant species that should be properly managed. Dichaetomyia corrugicerca and Phallosphaera gravelyi were newly recorded species. Specific control measures should be implemented in August and November when fly activities reached their peak.

【Abstract】 A specimen of Dichaetomyia corrugicerca ♂ was collected from Shanghai Jiangwan airport on October 2006. It was a new record in Shanghai area. The morphological characters of this species were described, and some simple statements were also given about the typical characteristics, larvae morphology, biology and distribution of the genus.

One male Tatera indica(Hardwicke,1807) was intercepted in container from Indian at Shanghai Waigaoqiao port.The species is the main host of bubonic plague and zoonotic cutaneous Leishmaniasis.There was no distribution and no interception record in China.Its morphological description and biology habit were given in the present paper.

Ophyra aenescens(Wiedemann,1830),which distributed in Palaearctic,Nearctic,Australia and Neotropic Regions,but not known to China before,was recently found in Shanghai,China.It was also intercepted from waste paper in a cargo from America and Australia.The present paper deals with the diagnostics,biology,distribution and others of the species.And the problem of intrusion of vector of medical importance is discussed.

specimens of Lispe were captured with hand-net during the investigation of flies at Shanghai Pudong International Airport from 2000 to 2002.According to identification,8 species of Lispe were found.They are Lispe assimilis,Lispe bivittata,Lispe consanguinea,Lispe hirsutipes,Lispe monochaita,Lispe orientalis,Lispe pygmaea,and Lispe tetrastigma,among them there were only 3 species: Lispe leucospila,Lispe orientalis,Lispe pygmaea in the previous record of Shanghai area,the rest being recorded firstly in Shanghai.And in China, Lispe tetrastigma has been found only in Taiwan province before.